There are hundreds of ways to create CO2 chambers. Given the diversity of what I do, I wanted options. What if I just need to put down a few pink mice? What if I have to put down an adult Burm? None of the plans that I saw really fit the bill for being so adaptable. So while trying to make one very versatile, I think i got a pretty simple one as well. In all, it costs about $50 with cheap refills.
The biggest expense is the CO2 tank. It is a 20 oz tank for Paintball that I got from Wal Mart for $28. That is because it is like Propane in that you have to have an empty tank to exchange. So if you already have an empty tank or pick one up cheap off Ebay, the cost is only about $8 to trade out for a full one.
Next i needed the connector hose. This is used by Paintballers as well to connect a large CO2 to the gun. At the Sports Store, they were $40, but I picked one up on Ebay for $14. It has 1/2" connectors and a dial to adjust air flow.
Those were the two big parts. Only a few left.
A Step Down adapter $4.50:
Hose Barb Adapter $2:
1/8" tubing $2.50 (WAYYYY to much tubing:
In all, it took about 90 seconds to assemble. I think it actually took longer to open the packages than to assemble th apparatus.
Now you can turn virtually any container into a CO2 Chamber. As an example, I just used a cheap food storage container. I drilled one hole (the size of the hose) on each side of the lid and popped the hose in one side for intake and the other is open for exhaust.
This size will work great for most rodents but I could just as easily hook it up to a trash can for large specimens.
Simple, versatile, and fits in a drawer.
Just be sure to open the valve slowly so the lid doesn't pop off the container (or rarely pop the tube off the barb). For most containers, you would never even open up the valve all the way. Just open it enough to get a good flow, then drop it down to a minimum after you think all the Oxygen as been pushed out to keep air from getting back in.
**Warning** Only use in a well ventilated area.
Couldn't let me have another week of feeling so confident that I made a how-to thread, huh? Just had to show me up? Now I am back to feeling like an RTB peon, lol. Man, this is worse than when Stargazer fixs a spelling error. 
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